Thioredoxin reductase

Thioredoxin Reductases (TR, TrxR) are the only known enzymes to reduce thioredoxin (Trx). Two classes of thioredoxin reductase have been identified: one class in bacteria and some eukaryotes and one in animals. Both classes are flavoproteins which function as homodimers. Each monomer contains a FAD prosthetic group, a NADPH binding domain, and an active site containing a redox-active disulfide bond.

Cellular Role
Thioredoxin reductase is the only enzyme known to catalyze the reduction of thioredoxin and hence is a central component in the thioredoxin system. Together with thioredoxin (Thx) and NADPH this system's most general description is as a method of forming reduced disulfide bonds in cells. Electrons are taken from NADPH via TrxR and are transferred to the active site of Trx, which goes on to reduce protein disulfides or other substrates. The Trx system exists in all living cells and has an evolutionary history tied to DNA as a genetic material, defense against oxidative damage due to oxygen metabolism, and redox signaling using molecules like hydrogen peroxide and nitric oxide.



Diversity
Two classes of thioredoxin reductase have evolved independently: These two classes of TrxR have only ~20% sequence identity in the section of primary sequence where they can be reliably aligned. The net reaction of both classes of TrxR is identical but the mechanism of action of each is distinct.
 * A high molecular weight (MW = ~55,000) type containing a selenocysteine residue in its active site has been identified in higher eukaryotes including humans. This TxR is related to glutathione reductase, trypanothione reductase, mercuric reductase and lipoamide dehydrogenase.
 * A low molecular weight (MW = ~ 35,000) type has been identified in archaea, bacteria and other eukarya.

Humans express three thioredoxin reductase isozymes: TrxR1 (cytosolic), TrxR2 (mitochondrial), TrxR3 (testis specific). Each isozyme is encoded by a separate gene:

Structure
E. coli thioredoxin reductase structure: In E. coli ThxR there is are two binding domains, one for FAD and another for NADPH. The connection between these two domains is a two-stranded anti-parallel f3-sheet. Each domain individually is very similar to the analogous domains in glutathione reductase, and lipoamide dehydrogenase but they relative orientation of these domains in ThxR is rotated by 66 degrees. This becomes significant in the enzyme mechanism of action which is described below. ThxR homo-dimerizes with the interface between the two monomers formed by three alpha-helicies and two loops. Each monomer can separately bind a molecule of thioredoxin.

Mechanism
E. coli thioredoxin reductase mechanism: In E. coli ThxR the spatial orientation of the FAD and NADPH domains are such that the redox-active rings of FAD and NADPH are not in close proximity to each other. When the FAD domain of E. coli is rotated 66 degrees with the NADPH domain remaining fixed the two prosthetic groups move into close contact allowing electrons to pass from NADPH to FAD and then to the active site disulfide bond. The conserved active site residues in E. coli are -Cys-Ala-Thr-Cys-.

Mammalian thioredoxin reductase mechanism: Mammalian TrxRs have a much higher sequence homology with glutathione reductase than E. coli. The active-site Cys residues in the FAD domain and bound NADPH domain are in close proximity removing the necessity for a 66 degree rotation for electron transfer found in E. coli. An additional feature of the mammalian mechanism is the presence of a selenocystine residue at the C-terminal end of the protein which is required for catalytic activity. The conserved residues in mammalian active site are -Cys-Val-Asn-Val-Gly-Cys-.



Clinical significance
- In cancer treatment: Since the activity of this enzyme is essential for cell growth and survival, it is a good target for anti-tumor therapy. Furthermore, the enzyme is upregulated in several types of cancer, including malignant mesothelioma. For example, motexafin gadolinium (MGd) is a new chemotherapeutic agent that selectively targets tumor cells, leading to cell death and apoptosis via inhibition of thioredoxin reductase and ribonucleotide reductase.

- In cardiomyopathy: Dilated cardiomyopathy (DCM) is a common diagnoses in cases of congestive heart failure. Thioredoxin reductases are essential proteins for regulating cellular redox balance and mitigating the damage caused by reactive oxygen species generated via oxidative phosphorylation in the mitochondria. Inactivation of mitochondrial TrxR2 in mice results in thinning of the ventricular heart walls and embryonic death. Furthermore two mutations in the TrxR2 gene are found in patients diagnosed with DCM and not in a control population. It is hypothesized that the pathological impact of these mutations is an impaired ability to control oxidative damage in cardiac myocytes.