SDHB

Succinate dehydrogenase [ubiquinone] iron-sulfur subunit, mitochondrial (SDHB) also known as iron-sulfur subunit of complex II (Ip) is a is_associated_with::protein that in humans is encoded by the SDHB is_associated_with::gene.

The is_associated_with::succinate dehydrogenase (also called SDH or Complex II) protein complex catalyzes the oxidation of succinate (succinate + ubiquinone => fumarate + ubiquinol). SDHB is one of four protein subunits forming succinate dehydrogenase, the other three being is_associated_with::SDHA, SDHC and is_associated_with::SDHD. The SDHB subunit is connected to the is_associated_with::SDHA subunit on the hydrophilic, catalytic end of the SDH complex. It is also connected to the SDHC/is_associated_with::SDHD subunits on the hydrophobic end of the complex anchored in the mitochondrial membrane. The subunit is an is_associated_with::iron-sulfur protein with three iron-sulfur clusters. It weighs 30 kDa.

Structure
The gene that codes for the SDHB protein is nuclear, not mitchondrial DNA. However, the expressed protein is located in the inner membrane of the is_associated_with::mitochondria. The location of the gene in humans is on the first chromosome at locus p36.1-p35. The is_associated_with::gene is coded in 1,162 base pairs, partitioned in 8 is_associated_with::exons. The expressed protein weighs 18.6 kDa and is composed of 180 amino acids. SDHB contains the iron-sulphur clusters necessary for tunneling electrons through the complex. It is located between is_associated_with::SDHA and the two is_associated_with::transmembrane subunits SDHC and is_associated_with::SDHD.

Function


The SDH complex is located on the inner membrane of the is_associated_with::mitochondria and participates in both the is_associated_with::Citric Acid Cycle and is_associated_with::Respiratory chain. SDHB acts as an intermediate in the basic SDH enzyme action shown in Figure 1:
 * 1) is_associated_with::SDHA converts is_associated_with::succinate to is_associated_with::fumarate as part of the is_associated_with::Citric Acid Cycle. This reaction also converts is_associated_with::FAD to FADH2.
 * 2) Electrons from the FADH2 are transferred to the SDHB subunit iron clusters [2Fe-2S],[4Fe-4S],[3Fe-4S].
 * 3) Finally the electrons are transferred to the is_associated_with::Ubiquinone (Q) pool via the SDHC/is_associated_with::SDHD subunits. This function is part of the is_associated_with::Respiratory chain.

Initially, is_associated_with::SDHA oxidizes is_associated_with::succinate via is_associated_with::deprotonation at the is_associated_with::FAD binding site, forming FADH2 and leaving is_associated_with::fumarate, loosely bound to the active site, free to exit the protein. Electrons from FADH2 are transferred to the SDHB subunit iron clusters [2Fe-2S],[4Fe-4S],[3Fe-4S] and tunnel along the [Fe-S] relay until they reach the [3Fe-4S] is_associated_with::iron sulfur cluster. The electrons are then transferred to an awaiting is_associated_with::ubiquinone molecule at the Q pool active site in the SDHC/is_associated_with::SDHD dimer. The O1 is_associated_with::carbonyl oxygen of ubiquinone is oriented at the active site (image 4) by is_associated_with::hydrogen bond interactions with Tyr83 of is_associated_with::SDHD. The presence of electrons in the [3Fe-4S] iron sulphur cluster induces the movement of ubiquinone into a second orientation. This facilitates a second hydrogen bond interaction between the O4 carbonyl group of ubiquinone and Ser27 of SDHC. Following the first single electron reduction step, a is_associated_with::semiquinone radical species is formed. The second electron arrives from the [3Fe-4S] cluster to provide full reduction of the ubiquinone to is_associated_with::ubiquinol.

Clinical significance
is_associated_with::Germline mutations in the gene can cause familial is_associated_with::paraganglioma (in old nomenclature, Paraganglioma Type PGL4). The same condition is often called familial is_associated_with::pheochromocytoma. Less frequently, is_associated_with::renal cell carcinoma can be caused by this mutation.

Paragangliomas related to SDHB mutations have a high rate of malignancy. When malignant, treatment is currently the same as for any malignant paraganglioma/pheochromocytoma.

Cancer
Paragangliomas caused by SDHB mutations have several distinguishing characteristics:


 * 1) Malignancy is common, ranging from 38%-83%  in carriers with disease. In contrast,  tumors caused by is_associated_with::SDHD mutations are almost always benign. Sporadic paragangliomas are malignant in less than 10% of cases.
 * 2) Malignant paragangliomas caused by SDHB are usually (perhaps 92% ) extra-adrenal. Sporadic pheochromocytomas/paragangliomas are extra-adrenal in less than 10% of cases.
 * 3) The is_associated_with::penetrance of the gene is often reported as 77% by age 50 (i.e. 77% of carriers will have at least one tumour by the age of 50). This is likely an overestimate. Currently (2011), families with silent SDHB mutations are being screened to determine the frequency of silent carriers.
 * 4) The average age of onset is approximately the same for SDHB vs non-SDHB related disease (approximately 36 years).

Mutations causing disease have been seen in is_associated_with::exons 1 through 7, but not 8. As with the SDHC and is_associated_with::SDHD genes, SDHB is a is_associated_with::tumor suppressor gene. Note the is_associated_with::SDHA gene is not a tumor suppressor gene.

Tumor formation generally follows the Knudson "two hit" hypothesis. The first copy of the gene is mutated in all cells, however the second copy functions normally. When the second copy mutates in a certain cell due to a random event, Loss of Heterozygosity (LOH) occurs and the SDHB protein is no longer produced. Tumor formation then becomes possible.

Given the fundamental nature of the SDH protein in all cellular function, it is not currently understood why only paraganglionic cells are affected. However, the sensitivity of these cells to oxygen levels may play a role.

Disease pathways
The precise pathway leading from SDHB mutation to tumorigenesis is not determined; there are several proposed mechanisms.

Generation of reactive oxygen species


When succinate-ubiquinone activity is inhibited, electrons that would normally transfer through the SDHB subunit to the Ubiquinone pool are instead transferred to O2 to create Reactive Oxygen Species (ROS) such as is_associated_with::superoxide. The dashed red arrow in Figure 2 shows this. ROS accumulate and stabilize the production of HIF1-α. HIF1-α combines with HIF1-β to form the stable HIF heterodimeric complex, in turn leading to the induction of antiapoptotic genes in the cell nucleus.

Succinate accumulation in the cytosol
SDH inactivation can block the oxidation of is_associated_with::succinate, starting a cascade of reactions:
 * 1) The succinate accumulated in the mitochondrial matrix  diffuses through the inner and outer mitochondrial membranes to the is_associated_with::cytosol (purple dashed arrows in  Figure 2).
 * 2) Under normal cellular function, HIF1-α in the cytosol is quickly hydroxylated by is_associated_with::prolyl hydroxylase (PHD), shown with the light blue arrow. This process is blocked by the accumulated succinate.
 * 3) HIF1-α stabilizes and passes to the cell nucleus (orange arrow) where it combines with HIF1-β to form an active HIF complex that induces the expression of tumor causing genes.

This pathway raises the possibility of a therapeutic treatment. The build-up of succinate inhibits PHD activity. PHD action normally requires oxygen and alpha-ketoglutarate as is_associated_with::cosubstrates and ferrous iron and ascorbate as cofactors. Succinate competes with α-ketoglutarate in binding to the PHD enzyme. Therefore, increasing α-ketoglutarate levels can offset the effect of succinate accumulation.

Normal α-ketoglutarate does not permeate cell walls efficiently, and it is necessary to create a cell permeating derivative (e.g. α-ketoglutarate esters). In-vitro trials show this supplementation approach can reduce HIF1-α levels, and may result in a therapeutic approach to tumours resulting from SDH deficiency.

Impaired developmental apoptosis
Paraganglionic tissue is derived from the is_associated_with::neural crest cells present in an is_associated_with::embryo. Abdominal extra-adrenal paraganglionic cells secrete catecholamines that play an important role in fetal development. After birth these cells usually die, a process that is triggered by a decline in is_associated_with::nerve growth factor (NGF)which initiates is_associated_with::apoptosis (cell death).

This cell death process is mediated by an enzyme called prolyl hydroxylase EglN3. Succinate accumulation caused by SDH inactivation inhibits the prolyl hydroxylase EglN3. The net result is that paranglionic tissue that would normally die after birth remains, and this tissue may be able to trigger paraganglioma/pheochromocytoma later.

Glycolysis upregulation
Inhibition of the Citric Acid Cycle forces the cell to create ATP glycolytically in order to generate its required energy. The induced glycolytic enzymes could potentially block cell apoptosis.

RNA editing
The mRNA transcripts of the SDHB gene in human are edited through an unknown mechanism at ORF nucleotide position 136 causing the conversion of C to U and thus generating a stop codon resulting in the translation of the edited transcripts to a truncated SDHB protein with an R46X amino acid change. This editing has been shown in is_associated_with::monocytes and some human lymphoid cell-lines, and is enhanced by is_associated_with::hypoxia.