IGFBP3

Insulin-like growth factor-binding protein 3, also known as IGFBP-3, is a is_associated_with::protein that in humans is encoded by the IGFBP3 is_associated_with::gene. IGFBP-3 is one of six IGF binding proteins (IGFBP-1 to IGFBP-6) that have highly conserved structures and bind the insulin-like growth factors is_associated_with::IGF-1 and is_associated_with::IGF-2 with high affinity. IGFBP-7, sometimes inappropriately included in this family, shares neither the conserved structural features nor the high IGF affinity.

Function
IGFBP-3 was first isolated, characterized, and quantitated in human plasma, in 1986. It has well-documented functions in the circulation, in the extracellular environment, and inside cells. It is the main IGF transport protein in the bloodstream, where it carries the growth factors predominantly in stable complexes that contain the binding protein, either IGF-1 or IGF-2, and a third protein called the acid-labile subunit or ALS.

For IGFs to reach the tissues from the bloodstream, the circulating complexes are believed to partly dissociate, possibly enhanced by limited proteolysis of IGFBP-3. The IGF-1/IGFBP-3 ratio has sometimes been used as an index of IGF bioavailability in the human circulation, but this ignores IGF-1 binding to other IGFBPs (so the ratio is affected by the concentrations of all six IGFBPs), and the fact that IGF-2, which is three times more abundant than IGF-1 in the bloodstream of adults, occupies the majority of binding sites on circulating IGFBP-3.

Within tissues, IGFBP-3 can bind IGF-1 and IGF-2 released by many cell types, and block their access to the IGF-1 receptor (is_associated_with::IGF1R), which is activated by both IGFs. IGFBP-3 also interacts with cell-surface proteins, affecting cell signaling from outside the cell or after internalization, and also enters the cell nucleus where it binds to nuclear hormone receptors and other ligands. High levels of IGFBP-3 within tumors are associated with increased cancer severity (or worse outcome) for some cancers, but decreased severity or better outcome for others. No cases of IGFBP3 gene deletion in humans have been reported, but mice lacking the gene show near-normal growth.

Gene and protein structure
The IGFBP3 gene (or IBP3), on human chromosome 7, is organized into four protein-coding is_associated_with::exons with a 5th exon in the 3’ untranslated region. It is located adjacent to the is_associated_with::IGFBP1 gene in tail-to-tail orientation, separated by 20 kb. The encoded protein includes a 27-residue is_associated_with::signal peptide followed by the 264-residue mature protein. IGFBP-3 shares with the other five high-affinity IGFBPs and a 3-domain structure:


 * 1) A conserved is_associated_with::N-terminal domain containing a cysteine rich region (12 cysteine residues) with multiple intra-domain is_associated_with::disulfide bonds, a IGFBP motif (GCGCCXXC), the primary site of IGF binding.
 * 2) A highly variable central or linker domain (only 15% conservation between IGFBPs).
 * 3) A conserved is_associated_with::C-terminal domain containing secondary IGF binding residues, a cysteine rich region (6 cysteine residues), an 18 residue basic motif that binds is_associated_with::heparin, the acid labile subunit (ALS), and a is_associated_with::nuclear localization sequence.

The linker domain is the site of most is_associated_with::post-translational modification, which include is_associated_with::glycosylation, is_associated_with::phosphorylation, and limited is_associated_with::proteolysis. By electrophoretic analysis IGFBP-3 appears as a doublet, owing to the occupancy of either two or three of its is_associated_with::N-glycosylation sites by carbohydrate. Hypoglycosylated IGFBP-3 may be seen after extended glucose starvation.

Many is_associated_with::proteases are known to cleave IGFBP-3 at single linker-domain sites, and in the circulation of pregnant women, IGFBP-3 is entirely proteolyzed, yet still capable of carrying normal amounts of IGF-1 and IGF-2. Binding capacity appears to be retained after proteolysis because of a cooperative interaction between the two proteolyzed fragments, that together maintain an active IGF-binding site.

Sites and regulation of production
IGFBP-3 mRNA is expressed in all tissue examined, with kidney, stomach, placenta, uterus and liver showing highest expression in rat tissues. Rat liver IGFBP-3 mRNA is found in nonparenchymal cells including sinusoidal endothelium, but not in is_associated_with::hepatocytes. In contrast, human hepatocytes do express IGFBP-3.

IGFBP-3 levels in human serum are, like IGF-1, dependent on is_associated_with::growth hormone (GH); for example, serum IGFBP-3 is increased in acromegaly and low in GH-deficient children. However, IGFBP-3 gene expression in human liver is GH-independent. Because it is stabilized in human serum by forming complexes with IGF-1 and ALS, which are both GH-dependent, serum IGFBP-3 also appears regulated by GH. Its production by some non-hepatic tissues may also be directly GH-regulated. is_associated_with::Immunoassays for serum IGFBP-3 are often used as part of the diagnosis of childhood GH-deficiency.

The most widely-studied IGFBP3 polymorphism, at nucleotide-202 in the promoter region, is significantly associated with circulating IGFBP-3 levels, although the mechanism is unclear. In some studies circulating IGFBP-3 also appears to be nutritionally regulated, although this may not be seen at the mRNA level. IGFBP-3 has been identified in human lymph, nipple aspirate, milk, amniotic fluid, follicular fluid, seminal plasma, urine, peritoneal dialysate, synovial fluid, tear fluid, and cerebrospinal fluid, in addition to serum.

Many factors increase IGFBP-3 production by cells, including is_associated_with::transforming growth factor-β (TGFβ), tumor necrosis factor-α, vitamin D, retinoic acid, IGF-1, and stimuli such as chemotherapy that activate the tumor suppressor p53. Estrogen inhibits IGFBP-3 production, and its tissue levels are lower in estrogen receptor (ER)-positive breast cancers than in ER-negative cancers.

Interactions
The main IGFBP-3 ligands in the circulation are IGF-1 and IGF-2, and the acid-labile subunit (ALS). The serum proteins is_associated_with::transferrin, is_associated_with::fibronectin, and is_associated_with::plasminogen are also known to bind IGFBP-3. In the cell and tissue environment many other interactions have been described (see Table). Two unrelated cell-surface proteins have been designated as IGFBP-3 receptors: low density lipoprotein receptor-related protein 1 (is_associated_with::LRP1), also known as alpha-2-macroglobulin receptor or type V TGFβ receptor and the transmembrane protein is_associated_with::TMEM219. Both are believed to mediate antiproliferative effects. Functional interactions with the is_associated_with::EGF receptor and the type I/type II TGFβ receptor system have also been reported, and other cell-surface proteins such as proteoglycans also bind IGFBP-3. IGFBP-3 can enter cells by both clathrin-mediated and caveolin-mediated endocytosis. possibly involving the transferrin receptor.

IGFBP-3 enters the cell nucleus by a mechanism that is incompletely understood, but involves its binding to importin-β. Within the nucleus, it can modulate is_associated_with::nuclear hormone receptor activity by direct binding to is_associated_with::retinoid X receptor, is_associated_with::retinoic acid receptor, is_associated_with::vitamin D receptor, is_associated_with::PPARγ, and is_associated_with::nur77, IGFBP-3 also interacts with DNA-dependent protein kinase within the nucleus to promote the repair of DNA damage.

Cellular actions
IGFBP-3 exerts is_associated_with::antiproliferative effects in many cell types by blocking the ability of is_associated_with::IGF-1 and is_associated_with::IGF-2 to activate the is_associated_with::IGF1R (which stimulates cell proliferation). For example, in esophageal is_associated_with::epithelial cells, responsiveness to IGF-1 stimulation is suppressed by secreted IGFBP-3 and restored when IGFBP-3 is downregulated by is_associated_with::epidermal growth factor. IGFBP-3 can also inhibit cell function by mechanisms that are independent of effects on IGF1R signaling, even in cells that entirely lack IGF1R. IGF (or IGF1R) independent effects are commonly studied using mutant forms of IGFBP-3 with decreased IGF binding affinity. Thus, IGFBP-3-induced apoptosis in differentiating is_associated_with::chondrocyte precursor cells is seen equally with a non-IGF binding IGFBP-3 mutant, demonstrating that the mechanism does not involve IGF binding. IGF1R-independent growth inhibition by IGFBP-3 may involve the induction of pro-apoptotic proteins such as Bax and Bad and may be mediated by ceramides (pro-is_associated_with::apoptotic lipids), or potentiate is_associated_with::ceramide action IGFBP-3 interaction with nuclear hormone receptors may also lead to inhibition of cell proliferation. Contrasting with the typical growth-inhibitory effects of IGFBP-3, stimulation of cell proliferation by IGFBP-3 has also been observed. This can occur either by enhancing IGF-stimulated proliferation or in the absence of IGF-1. In endothelial cells and mammary epithelial cells, the stimulatory effect of IGFBP-3 has been shown to involve activation of the enzyme is_associated_with::sphingosine kinase, and generation of the bioactive lipid, is_associated_with::sphingosine-1-phosphate, which promotes growth by transactivating the EGFR receptor.

Role in cancer
Based on cell growth experiments, animal cancer models, and is_associated_with::epidemiological studies, it appears that IGFBP-3 functions as a low-is_associated_with::penetrance is_associated_with::tumor suppressor gene.

Dysregulation of IGFBP-3 has been implicated in many cancers. IGFBP-3 is sometimes referred to as a is_associated_with::tumor suppressor, and downregulation of its tissue expression by promoter is_associated_with::hypermethylation in some cancers, such as is_associated_with::hepatoma. and is_associated_with::non-small cell lung cancer may be associated with poor patient outcome. However, consistent with the dual inhibitory and stimulatory roles of IGFBP-3 seen in cell culture, there are other cancer types, such as breast cancer, pancreatic cancer, and clear cell is_associated_with::renal cell cancer in which high tissue IGFBP-3 expression has been linked to poor prognostic features or patient outcome. The mechanisms regulating these contrasting effects of IGFBP-3 in vivo are not well understood.

Since IGFBP-3 is abundant in the bloodstream of healthy adults (typically 2–4 mg/L), and is largely stabilized by its complex formation with IGFs and ALS, it is unlikely that tumor-derived IGFBP-3 has a large influence on circulating levels. There have been many studies linking circulating IGFBP-3 levels to the presence, or risk, of various cancers, or to patient outcomes. but unequivocal conclusions have often been lacking. For example, high plasma IGFBP-3 levels were associated with a reduced prospective risk of colorectal cancer in women. but in a study including men and women, colon cancer risk was positively associated with plasma IGFBP-3, while there was no significant association for rectal cancer. A large systematic review concluded that circulating IGFBP-3 levels showed a modest association with increased risk for a number of cancers, but the results vary among sites.

IGFBP-3 protein levels decrease during the progression of is_associated_with::prostate cancer from benign to metastatic disease although production of the protein does not cease completely. IGFBP-3 is still made (at a lower level) by prostate cancer cells and secreted into the surrounding environment. However, instead of the full length, functional protein, IGFBP-3 is found to be cleaved. This decreases the affinity of IGF binding to IGFBP-3, making the growth factors more likely to bind the IGF1R and promote cell survival.

Table: IGFBP-3 binding partners
IGFBP3 has been shown to interact with:


 * is_associated_with::ADAM12
 * is_associated_with::ADAM28
 * COL1A1
 * FN1
 * IGFALS
 * IGF1,
 * IGF2
 * HSPA5
 * PLG
 * RXRA
 * TF
 * KPNB1
 * PRKDC
 * EGFR
 * is_associated_with::LTBP1