Prolyl hydroxylase

Prolyl hydroxylase (or procollagen-proline dioxygenase) is an enzyme involved in the production of collagen, acting to hydroxylate proline to hydroxyproline.

It requires vitamin C as a cofactor to keep Iron in the reduced state (Fe2+) but it is not stoichiometrically consumed. α-ketoglutarate serves as reducing agent.

Reaction: Proline + α-ketoglutarate + O2 → 4-hydroxyproline + CO2 + succinate

It is classified under.