SENP1

Sentrin-specific protease 1 is an is_associated_with::enzyme that in humans is encoded by the SENP1 is_associated_with::gene.

General
So far there are seven SUMO is_associated_with::proteases in humans that have been designated SENP1-7 (sentrin/SUMO-specific protease).1 The seven proteases possess a conserved is_associated_with::C-terminal domain which are variable in size, and with a distinct is_associated_with::N-terminal domain between them. The C-terminal domain shows is_associated_with::catalytic activity and N-terminal domain regulates cell localization and substrate specificity.

Features
SENP1 (Sentrin-specific protease 1) is a human protease of 643 amino acids with a weight of 73 kDa, is_associated_with::EC number in humans 3.4.22.B70, which adopts a conformation that identifies it as a member of the superfamily of cysteine proteases contain a catalytic triad with characterized three amino acids: a is_associated_with::cysteine at position 602, a is_associated_with::histidine at position 533 and is_associated_with::aspartic acid at position 550. The important nucleophile is cysteine located at the N-terminal is_associated_with::alpha helix of the protein core, the other two amino acids, aspartate and histidine, are located in a is_associated_with::beta sheet end.

Location
Both SENP1 are located in the nucleus and is_associated_with::cytosol depending on the cell type, although it has been seen that is exported out from the nucleus to the cytosol through a sequence of nuclear export (NES) that is located at the C-terminus. The mammalian SENP1 is localized mainly in the nucleus.

Function
SENP1 catalyzes maturation is_associated_with::SUMO protein (small ubiquitin-related modifier), which causes hydrolysis peptide bond of SUMO is in a conserved sequence Gly-Gly-|-Ala-Thr-Tyr at the C-terminal to be added to the conjugation of other proteins (is_associated_with::sumoylation). In vertebrates there are three members of the family of SUMO: SUMO-1, -2 and -3. SENP1 can catalyze any of these three. This conjugation of SUMO toward other proteins is a lot like ubiquitination, however these modifications leads to different results depending on the type of protein been modified.