RAD18

E3 ubiquitin-protein ligase RAD18 is an is_associated_with::enzyme that in humans is encoded by the RAD18 is_associated_with::gene.

Animal models
is_associated_with::Model organisms have been used in the study of RAD18 function. A conditional is_associated_with::knockout mouse line, called Rad18tm1a(EUCOMM)Wtsi, was generated as part of the is_associated_with::EUCOMM program — a high-throughput is_associated_with::mutagenesis project to generate and distribute animal models of disease to interested scientists — at the is_associated_with::Wellcome Trust Sanger Institute. Mice lacking Rad18 had no significant defects in viability or is_associated_with::fertility, therefore  male and female animals underwent a standardized is_associated_with::phenotypic screen to determine the effects of deletion.

Twenty five tests were carried out and four significant is_associated_with::phenotypes were reported:


 * is_associated_with::Mutant male mice had a decreased is_associated_with::body weight compared to is_associated_with::wildtype control mice.
 * Mutant male mice showed increased activity, is_associated_with::VO2 and is_associated_with::energy expenditure, determined by is_associated_with::indirect calorimetry.
 * is_associated_with::Dual-energy X-ray absorptiometry (DEXA) showed mutant male mice had a decrease in is_associated_with::fat mass.
 * A is_associated_with::micronucleus test found a potential increase in is_associated_with::DNA damage in mutant mice.

Interactions
RAD18 has been shown to interact with is_associated_with::HLTF, is_associated_with::UBE2B and is_associated_with::UBE2A.