Flap structure-specific endonuclease 1

Flap endonuclease 1 is an is_associated_with::enzyme that in humans is encoded by the FEN1 is_associated_with::gene.

Function
The protein encoded by this gene removes 5' overhanging "flaps" (or short sections of single stranded DNA that "hang off" because their nucleotide bases are prevented from binding to their complementary base pair—despite any base pairing downstream) in DNA repair and processes the 5' ends of is_associated_with::Okazaki fragments in lagging strand DNA synthesis. Direct physical interaction between this protein and AP endonuclease 1 during long-patch base excision repair provides coordinated loading of the proteins onto the substrate, thus passing the substrate from one enzyme to another. The protein is a member of the XPG/RAD2 endonuclease family and is one of ten proteins essential for cell-free DNA replication. DNA secondary structure can inhibit flap processing at certain is_associated_with::trinucleotide repeats in a length-dependent manner by concealing the 5' end of the flap that is necessary for both binding and cleavage by the protein encoded by this gene. Therefore, secondary structure can deter the protective function of this protein, leading to site-specific trinucleotide expansions.

Interactions
Flap structure-specific endonuclease 1 has been shown to interact with:


 * is_associated_with::APEX1,
 * BLM
 * CDK2,
 * CCNA2,
 * is_associated_with::EP300,
 * HNRNPA1,
 * is_associated_with::PCNA,     and
 * WRN.

Over expression of FEN1 in cancers
FEN1 is over-expressed in the majority of cancers of the breast, prostate, stomach, neuroblastomas, pancreatic, and lung.

FEN1 is an essential enzyme in an inaccurate pathway for repair of double-strand breaks in DNA called microhomology-dependent alternative end joining or is_associated_with::microhomology-mediated end joining (MMEJ). MMEJ always involves at least a small deletion, so that it is a mutagenic pathway. Several other pathways can also repair double-strand breaks in DNA, including the less inaccurate pathway of is_associated_with::non-homologous end joining (NHEJ) and accurate pathways using is_associated_with::homologous recombinational repair (HRR). Various factors determine which pathway will be used for repair of double strand breaks in DNA. When FEN1 is over-expressed (this occurs when its promoter is hypomethylated ) the highly inaccurate MMEJ pathway may be favored, causing a higher rate of mutation and increased risk of cancer.

Cancers are very often deficient in expression of one or more DNA repair genes, but over-expression of a DNA repair gene is unusual in cancer. For instance, at least 36 DNA repair enzymes, when mutationally defective in germ line cells, cause increased risk of cancer (hereditary is_associated_with::cancer syndromes). Similarly, at least 12 DNA repair genes have frequently been found to be epigenetically repressed in one or more cancers. (See also Epigenetically reduced DNA repair and cancer.) Ordinarily, deficient expression of a DNA repair enzyme results in increased un-repaired DNA damages which, through replication errors (translesion synthesis), lead to mutations and cancer. However, FEN1 mediated MMEJ repair is highly inaccurate, so in this case, over-expression, rather than under-expression, leads to cancer.