Subventricular zone



The subventricular zone (SVZ) is a paired brain structure situated throughout the lateral walls of the lateral ventricles. It has been associated with having four distinct layers of variable thickness and cell density, as well as cellular composition.

Layer I
The innermost layer (Layer I) contains a single layer (monolayer) of ependymal cells lining the ventricular cavity; these cells possess apical cilia and several basal expansions that may stand in either parallel or perpendicular to the ventricular surface. These expansions may interact intimately with the astrocytic processes that are interconnected with the hypocellular layer (Layer II).

Layer II
The secondary layer (Layer II) provides for a hypocellular gap abutting the former and has been shown to contain a network of functionally-correlated Glial Fibrillary Acid Protein (GFAP)-positive astrocytic processes that are linked to junctional complexes, yet lack cell bodes except for the rare neuronal stomata. While the function of this layer is yet unknown in humans, it has been hypothesized that the astrocytic and ependymal interconnections of Layer I and II may act to regulate neuronal functions, establish metabolic homeostasis, and/or control neuronal stem cell proliferation and differentiation during development. Potentially, such characteristics of the layer may act as a remainder of early developmental life or pathway for cellular migration given similarity to a homologous layer in bovine SVZ shown to have migratory cells common only to higher order mammals.

Layer III
The third layer (Layer III) forms a ribbon of astrocyte cell bodies that are believed to maintain a subpopulation of astrocytes able to proliferate in vivo and form multipotent neurospheres of self-renewal abilities in vitro. While some oligodendrocytes and ependymal cells have been found within the ribbon, they not only serve an unknown function, they are uncommon by comparison to the population of astrocytes that reside in the layer. The astrocytes present in Layer III can be divided into three populations through electron microscopy, with no unique functions yet recognizable; the first type is a small astrocyte of long, horizontal, tangential projections mostly found in Layer II; the second type is found between Layers II and III as well as within the astrocyte ribbon, characterized by its large size and many organelles; the third type is typically found in the lateral ventricles just above the hippocampus and is similar in size to the second type but contains few organelles.

Layer IV
The fourth and final layer (Layer IV) serves as a transition zone between Layer III with its ribbon of astrocytes and the brain parenchyma. It is identified by a high presence of myelin in the region.

Cell types
Four cell types are described in the SVZ:

1. Ciliated Ependymal Cells (Type E): are positioned facing the lumen of the ventricle, and function to circulate the cerebrospinal fluid.

2. Proliferating Neuroblasts (Type A): express PSA-NCAM, Tuj1, and Hu, and migrate in line order to the Olfactory Bulb

3. Slow Proliferating Cells (Type B): express Nestin and GFAP, and function to ensheathe migrating Type A Neuroblasts

4. Actively Proliferating Cells or Transit Amplifying Progenitors (Type C): express Nestin, and form clusters interspaced among chains throughout region

Function
The SVZ is a known site of neurogenesis and self-renewing neurons in the adult brain, serving as such given the interacting cell types and extracellular molecules promoting such cellular proliferation. Along with the subgranular zone of dentate gyrus, the subventricular zone serves as a source of neural stem cells in the process of adult neurogenesis. It harbors the largest population of proliferating cells in the adult brain of rodents, monkeys and humans.

While it has yet to have been studied in-depth in the human brain, the SVZ function in the rodent brain has been, to a certain extent, examined and defined for its abilities. With such research, it has been found that the dual-functioning astrocyte is the dominant cell in the rodent SVZ; this astrocyte acts as not only a neuronal stem cell, but also as a supporting cell that promotes neurogenesis through interaction with other cells. This function is also induced by microglia and endothelial cells that interact cooperatively with neuronal stem cells to promote neurogenesis in vitro, as well as extracellular matrix components such as teneascin-C (helps define boundaries for interaction) and Lewis X (binds growth and signaling factors to neural precursors). The human SVZ is different, however, from the rodent SVZ in two distinct ways; the first is that the astrocytes of humans are not in close juxtaposition to the ependymal layer, rather separated by a layer lacking cell bodies; the second is that the human SVZ lacks chains of migrating neuroblasts seen in rodent SVZ, in turn providing for a lesser number of neuronal cells in the human than the rodent. For this reason, while rodent SVZ proves as a valuable source of information regarding the SVZ and its structure-to-function relationship, the human model will prove significantly different.

In addition, some current theories propose that the SVZ may also serve as a site of proliferation for brain tumor stem cells (BTSCs), which are similar to neural stem cells in their structure and ability to differentiate into neurons, astrocytes, and oligodendrocytes. Studies have confirmed that a small population of BTSCs can not only produce tumors, but they can also maintain it through innate self-renewal and multipotent abilities. While this does not allow for inference that BTSCs arise from neural stem cells, it does raise an interesting question as to the relationship that exists from our own cells to those that can cause so much damage.

Current research
There are currently many different aspects of the SVZ being researched by individuals in the public and private sectors. Such research interests range from the role of the SVZ in neurogenesis, directed neuronal migration, to the previously mentioned tumorigenesis, as well as many others. Below there are summaries of the work of three different lab groups focusing primarily on one aspect of the SVZ; these include the role of SVZ in cell replacement after brain injury, simulation of NSC proliferation, and role in various tumorigenic cancers.

Roles of the mammalian subventricular zone in cell replacement after brain injury
Michael J. Romankob, Radoslaw Rolac, John R. Fikec,d, Francis G. Szelee, Maria L.V. Dizone, Ryan J. Fellingb, Christine Y. Brazelf, Steven W. Levisona,b,*. (2004). Progress in Neurobiology (74). 77-99.

Romankob et al., in an attempt to observe various cause and effect relationships on the SVZ due to potentially hazardous incidence upon the brain, describe the many experiments that were conducted to determine such outcomes. As was determined, the multitude of cells that exist in the SVZ were found to be somewhat vulnerable to their exposure agents, but affected only to the extent by which observations can be made regarding replacement of dead brain cells.

The effects of irradiation on the SVZ provided for a recognition of the amount or dose of radiation that can be given is determined mostly by the tolerance of the normal cells near the tumor. As described, the increasing dose of radiation and age led to decrease in three cell types of the SVZ, yet repair capacity of the SVZ was observed given lack of white matter necrosis; this stands for the fact that the SVZ was able to gradually replace the neuroglia of the brain. Chemotherapeutics were also tested for there effects on the SVZ, as they are currently used for many diseases yet provide for complications with the central nervous system. To do so, methotrexate (MTX) was used alone and in combination with radiation to find that roughly 70% of the total nuclear density of the SVZ had been depleted, yet given loss of neuroblast cells (progenitor cells), it was remarkable to find that SVZ NSCs would still generate neurospheres similar to subjects that did not receive such treatment. In relation to interruption of blood supply to the brain, cerebral hypoxia/ischemia (H/I) was found to also decrease the cell count of the SVZ by 20%, with 50% of neurons in the striatum and neocortex being destroyed., but the cell types of the SVZ killed were as non-uniform as the region itself. Upon subsequent testing, it was found that a different portion of each cell was eliminated, yet the medial SVZ cell population remained mostly alive. This may provide for a certain resiliency of such cells, with the uncommitted progenitor cells acting as the proliferating population following ischemia. Mechanical brain injury also induces cell migration and proliferation, as was observed in rodents, and it may also increase cell number, negating the previously held notion that no new neuronal cells can be generated.

In conclusion, this group was able to determine that cells in the SVZ are able to produce new neurons and glia throughout life, given it does not suffer damage as it is sensitive to any deleterious effects. Therefore, the SVZ can recover itself following mild injury, and potentially provide for replacement cell therapy to other affected regions of the brain.

Neuropeptide Y stimulates proliferation, migration and differentiation of neural precursors from the subventricular zone in adult mice
Decressac, M., Prestoz, L., Veran, J., Cantereau, A., Jaber, M., & Gaillard, A. (2009).Neurobiology of Disease, 34(3), 441-449.

In an attempt to characterize and analyze the mechanism concerning the proliferation of neuronal cells within the subventricular zone, Decressac et al. observed the proliferation of neural precursors in the mouse subventricular zone through injection of the neuropeptide Y (NPY). NPY is a commonly expressed protein of the central nervous system that has previously been shown to stimulate proliferation of neuronal cells in the olfactory epithelium and hippocampus. The peptide’s effects were observed through BrdU labeling and cell phenotyping that provided evidence for the migration of neuroblasts through the rostral migratory stream to the olfactory bulb (confirming previous experiments) and to the striatum. Such data supports the author’s hypothesis in that neurogenesis would be stimulated through introduction of such a peptide.

As NPY is a 36 amino acid peptide associated with many physiological and pathological conditions, it has multiple receptors that are broadly expressed in the developing and mature rodent brain. However, given in vivo studies performed by this group, the Y1 receptor displayed specifically mediated neuroproliferative effects through the induction of NPY with increased expression in the subventricular zone. Identification of the Y1 receptor also sheds light on the fact that the phenotype of expressed cells from such mitotic events are actually cells that are DCX+ (neuroblasts that migrate directly to the striatum) type. Along with the effects of NPY injection on striatal dopamine, GABA and glutamate parameters to regulate neurogenesis in the subventricular zone (previous study), this finding is still under consideration as it could be a secondary modulator of the aforementioned neurotransmitters.

As is necessary for all research, this group conducted its experiments with a broad perspective on the application of their findings, which they claimed could potentially benefit potential candidates for endogenous brain repair through stimulation of the subventricular zone neural stem cell proliferation. This natural molecular regulation of adult neurogenesis would be adjunct with therapies of appropriate molecules such as the tested NPY and Y1 receptor, in addition to pharmacological derivatives, in providing for manageable forms of neurodegenerative disorders of the striatal area.

The human subventricular zone: A source of new cells and a potential source of brain tumors
Alfredo Quiñones-Hinojosa, Kaisorn Chaichana

In an attempt to characterize the role of the subventricular zone in potential tumorigenesis, Quinones-Hinojosa et al. found that brain tumor stem cells (BTSCs) are stem cells that can be isolated from brain tumors by similar assays used for neuronal stem cells. In forming clonal spheres similar to neurospheres of neuronal stem cells, these BTSCs were able to differentiate into neurons, astrocytes and oligodendrocytes in vitro, yet more importantly capable of initiating tumors at low cell concentrations, providing a self-renewal capacity. It was therefore proposed that a small population of BTSCs with such self-renewal capabilities were maintaining tumors in diseases such as leukemia and breast cancer.

Several characterizing factors lead to the proposed idea of neuronal stem cells (NSCs) being the origin for BTSCs, as they share several features. These features are shown in the figure.

This group provides evidence of the SVZ’s apparent role in tumorigenesis as demonstrated by the possession of mitogenic receptors and their response to mitogenic stimulation, specifically type C cells that express the epidermal growth factor receptor (EGFR), making them highly proliferative and invasive. Additionally, the existence of microglia and endothelial cells within the SVZ was found to enhance neurogenesis, as well as providing for some directional migration of neuroblasts from the SVZ.

In following, while the SVZ has not yet been characterized as an exact site of tumorigenesis, it is distinctly possible that such a relationship exists between the NSC generation of the region and the consistently self-renewing cells of primary tumors that give way to secondary tumors once removed or irradiated.

Medical applications
Given the understanding of the SVZ as a region of neurogenesis for neuronal stem cells and its potential role in replacement cell therapy for damaged regions of the brain, the SVZ has proven and will continue to act as an important insight into cell therapy for many traumatic brain injuries. Current research is being conducted with aims of not only understanding the migratory and regulatory pathways of the neuronal stem cells and their progenitors, but also how to induce and potentially direct them in a desired direction.

While such research has not yet provided for any implementation of these findings, the future may hold possible treatments, chemical or otherwise, that will help those in need of brain cell replacement.