Cryptochrome

Cryptochromes (from the Greek κρυπτό χρώμα, hidden colour) are a class of is_associated_with::flavoproteins that are sensitive to blue light. They are found in is_associated_with::plants and is_associated_with::animals. Cryptochromes are involved in the is_associated_with::circadian rhythms of plants and animals, and in the sensing of magnetic fields in a number of species. The name Cryptochrome was proposed as a pun combining the cryptic nature of the photoreceptor, and the is_associated_with::cryptogamic organisms on which many blue light studies were carried out.

The two is_associated_with::genes Cry1 and Cry2 code for the two cryptochrome is_associated_with::proteins is_associated_with::CRY1 and is_associated_with::CRY2. In is_associated_with::insects and plants, CRY1 regulates the is_associated_with::circadian clock in a light-dependent fashion, whereas, in is_associated_with::mammals, CRY1 and CRY2 act as light-independent inhibitors of is_associated_with::CLOCK-BMAL1 components of the circadian clock. In plants, blue light photoreception can be used to cue developmental signals.

Discovery
Although is_associated_with::Charles Darwin first documented plant responses to blue light in the 1800s, it was not until the 1980s that research began to identify the pigment responsible. In 1980, researchers discovered that the HY4 gene of the plant is_associated_with::Arabidopsis thaliana was necessary for the plant's blue light sensitivity, and, when the gene was sequenced in 1993, it showed high sequence homology with is_associated_with::photolyase, a DNA repair protein activated by blue light. By 1995, it became clear that the products of the HY4 gene and its two human homologs did not exhibit photolyase activity and were instead a new class of blue light photoreceptor hypothesized to be circadian is_associated_with::photopigments. In 1996 and 1998, Cry homologs were identified in is_associated_with::Drosophila and mice, respectively.

Evolutionary history and structure
Cryptochromes (CRY1, CRY2) are evolutionarily old and highly conserved proteins that belong to the flavoproteins superfamily that exists in all kingdoms of life. All members of this superfamily have the characteristics of an N-terminal photolyase homology (PHR) domain. The PHR domain can bind to the is_associated_with::flavin adenine dinucleotide (FAD) cofactor and a light-harvesting is_associated_with::chromophore. Cryptochromes are derived from and closely related to photolyases, which are bacterial enzymes that are activated by light and involved in the repair of UV-induced DNA damage. In eukaryotes, cryptochromes no longer retain this original enzymatic activity. The structure of cryptochrome involves a fold very similar to that of photolyase, with a single molecule of FAD noncovalently bound to the protein. These proteins have variable lengths and surfaces on the C-terminal end, due to the changes in genome and appearance that result from the lack of DNA repair enzymes. The is_associated_with::Ramachandran plot shows that the is_associated_with::secondary structure of the CRY1 protein is primarily a right-handed is_associated_with::alpha helix with little to no steric overlap. The structure of CRY1 is almost entirely made up of alpha helices, with several loops and few is_associated_with::beta sheets. The molecule is arranged as an orthogonal bundle.

Phototropism
In plants, cryptochromes mediate is_associated_with::phototropism, or directional growth toward a light source, in response to blue light. This response is now known to have its own set of photoreceptors, the is_associated_with::phototropins. Unlike is_associated_with::phytochromes and phototropins, cryptochromes are not is_associated_with::kinases. Their flavin chromophore is reduced by light and transported into the cell nucleus, where it affects the is_associated_with::turgor pressure and causes subsequent stem elongation. To be specific, Cry2 is responsible for blue-light-mediated is_associated_with::cotyledon and leaf expansion. Cry2 overexpression in is_associated_with::transgenic plants increases blue-light-stimulated cotyledon expansion, which results in many broad leaves and no flowers rather than a few primary leaves with a flower. A double loss-of-function mutation in Arabidopsis thaliana Early Flowering 3 (elf3) and Cry2 genes delays flowering under continuous light and was shown to accelerate it during long and short days, which suggests that Arabidopsis CRY2 may play a role in accelerating flowering time during continuous light.

In the is_associated_with::sponge eyes, blue-light-receptive cryptochrome is also expressed. Most animals have some form of visual structure that allowed them to navigate the world, from simple eyespots up to complex refractive and compound eyes. The eyes utilize photo-sensitive is_associated_with::opsin proteins expressed in neurons to communicate information of the light environment to the nervous system, whereas sponge larvae use pigment ring eyes to mediate phototactic swimming. However, despite possessing many other is_associated_with::G-protein-coupled receptors (GPCRs), the fully sequenced genome of Amphimedon queenslandica, a demosponge larvae, lacks one vital visual component: a gene for a light-sensitive opsin pigment – which is essential for vision in other animals – suggesting that the sponge’s unique eyes might have evolved a completely novel light-detection mechanism. By using RNA probes, Todd Oakley research group determined that one of the two cryptochromes, Aq-Cry2, was produced near the sponge’s simple eye cells. Aq-Cry2 lacks photolyase activity and contains a flavin-based co-factor that is responsive to wavelengths of light that also mediate larval photic behavior. Defined as opsin-clade GPCRs, it possess a conserved Shiff base lysine that is central to opsin function. Like other sponges, A. queenslandica lacks a nervous system. This indicates that opsin-less sponge eyes utilize cryptochrome, along with other proteins, to direct or act in eye-mediated phototactic behavior. Therefore, A. queenslandica pigment ring eyes likely evolved convergently in the absence of opsins and nervous systems, and probably use as-yet-unknown molecular mechanisms that are fundamentally different from those employed by other animal eyes.

Light capture
Despite much research on the topic, cryptochrome is_associated_with::photoreception and is_associated_with::phototransduction in is_associated_with::Drosophila and Arabidopsis thaliana is still poorly understood. Cryptochromes are known to possess two chromophores: is_associated_with::pterin (in the form of is_associated_with::5,10-methenyltetrahydrofolic acid (MTHF)) and flavin (in the form of FAD). Both may absorb a is_associated_with::photon, and in Arabidopsis, pterin appears to absorb at a wavelength of 380 nm and flavin at 450 nm. Past studies have supported a model by which energy captured by pterin is transferred to flavin. Under this model of phototransduction, FAD would then be reduced to FADH, which probably mediates the is_associated_with::phosphorylation of a certain domain in cryptochrome. This could then trigger a is_associated_with::signal transduction chain, possibly affecting is_associated_with::gene regulation in the cell nucleus.

A new hypothesis proposes that in plant cryptochromes, the transduction of the light signal into a chemical signal that might be sensed by partner molecules could be triggered by a photo-induced negative charge within the protein - on the FAD cofactor or on the neighbouring aspartic acid. This negative charge would electrostatically repel the protein-bound ATP molecule and thereby also the protein C-terminal domain, which covers the ATP binding pocket prior to photon absorption. The resulting change in protein conformation could lead to phosphorylation of previously inaccessible phosphorylation sites on the C-terminus and the given phosphorylated segment could then liberate the transcription factor HY5 by competing for the same binding site at the negative regulator of photomorphogenesis is_associated_with::COP1.

A different mechanism may function in Drosophila. The true ground state of the flavin cofactor in Drosophila CRY is still debated, with some models indicating that the FAD is in an oxidized form, while others support a model in which the flavin cofactor exists in is_associated_with::anion radical form, •. Recently, researchers have observed that oxidized FAD is readily reduced to • by light. Furthermore, mutations that blocked photoreduction had no effect on light-induced degradation of CRY, while mutations that altered the stability of • destroyed CRY photoreceptor function. These observations provide support for a ground state of •. Researchers have also recently proposed a model in which is excited to its doublet or quartet state by absorption of a photon, which then leads to a conformational change in the CRY protein.

Circadian rhythm
Studies in animals and plants suggest that cryptochromes play a pivotal role in the generation and maintenance of circadian rhythms. In Drosophila, cryptochrome (dCRY) acts as a blue-light photoreceptor that directly modulates light input into the circadian clock, while in mammals, cryptochromes (CRY1 and CRY2) act as transcription is_associated_with::repressors within the circadian clockwork. Some insects, including the is_associated_with::monarch butterfly, have both a mammal-like and a Drosophila-like version of cryptochrome, providing evidence for an ancestral clock mechanism involving both light-sensing and transcriptional-repression roles for cryptochrome.

Cry is_associated_with::mutants have altered circadian rhythms, showing that Cry affects the circadian pacemaker. Drosophila with mutated Cry exhibit little to no mRNA cycling. A point mutation in cryb, which is required for flavin association in CRY protein, results in no PER or TIM protein cycling in either DD or LD. In addition, mice lacking Cry1 or Cry2 genes exhibit differentially altered free running periods, but are still capable of is_associated_with::photoentrainment. However, mice that lack both Cry1 and Cry2 are arrhythmic in both LD and DD and always have high Per1 mRNA levels. These results suggest that cryptochromes play a photoreceptive role, as well as acting as negative regulators of Per gene expression in mice.

In Drosophila
In Drosophila, cryptochrome functions as a blue light photoreceptor. Exposure to blue light induces a conformation similar to that of the always-active CRY mutant with a C-terminal deletion (CRYΔ). The half-life of this conformation is 15 minutes in the dark and facilitates the binding of CRY to other clock gene products, PER and TIM, in a light-dependent manner. Once bound by dCRY, dTIM is committed to degradation by the ubiquitin-is_associated_with::proteasome system.

Although light pulses do not entrain, full photoperiod LD cycles can still drive cycling in the is_associated_with::ventral-lateral neurons in the Drosophila brain. These data along with other results suggest that CRY is the cell-autonomous photoreceptor for body clocks in Drosophila and may play a role in nonparametric entrainment (entrainment by short discrete light pulses). However, the lateral neurons receive light information through both the blue light CRY pathway and the is_associated_with::rhodopsin pathway. Therefore, CRY is involved in light perception and is an input to the circadian clock, however it is not the only input for light information, as a sustained rhythm has been shown in the absence of the CRY pathway, in which it is believed that the rhodopsin pathway is providing some light input. Recently, it has also been shown that there is a CRY-mediated light response that is independent of the classical circadian CRY-TIM interaction. This mechanism is believed to require a flavin is_associated_with::redox-based mechanism that is dependent on potassium channel conductance. This CRY-mediated light response has been shown to increase is_associated_with::action potential firing within seconds of a light response in is_associated_with::opsin-knockout Drosophila.

Cryptochrome, like many genes involved in circadian rhythm, shows circadian cycling in is_associated_with::mRNA and protein levels. In Drosophila, Cry mRNA concentrations cycle under a light-dark cycle (LD), with high levels in light and low levels in the dark. This cycling persists in constant darkness (DD), but with decreased amplitude. The transcription of the Cry gene also cycles with a similar trend. CRY protein levels, however, cycle in a different manner than Cry transcription and mRNA levels. In LD, CRY protein has low levels in light and high levels in dark, and, in DD, CRY levels increase continuously throughout the subjective day and night. Thus, CRY expression is regulated by the clock at the transcriptional level and by light at the translational and posttranslational level.

Overexpression of Cry also affects circadian light responses. In Drosophila, Cry overexpression increases flies’ sensitivity to low-intensity light. This light regulation of CRY protein levels suggests that CRY has a circadian role upstream of other clock genes and components.

In mammals
Cryptochrome is one of the four groups of mammalian clock genes/proteins that generate a transcription-translation negative-feedback loop (TTFL), along with Period (PER), CLOCK, and BMAL1. In this loop, CLOCK and BMAL1 proteins are is_associated_with::transcriptional activators, which together bind to the promoters of the Cry and Per genes and activate their transcription. The CRY and PER proteins then bind to each other, enter the nucleus, and inhibit CLOCK-BMAL1-activated transcription.

In mice, Cry1 expression displays circadian rhythms in the is_associated_with::suprachiasmatic nucleus, a brain region involved in the generation of circadian rhythms, with mRNA levels peaking during the light phase and reaching a minimum in the dark. These daily oscillations in expression are maintained in constant darkness.

While CRY has been well established as a TIM homolog in mammals, the role of CRY as a photoreceptor in mammals has been controversial. Early papers indicated that CRY has both light-independent and -dependent functions. A study in 2000 indicated that mice without rhodopsin but with cryptochrome still respond to light; however, in mice without either rhodopsin or cryptochrome, is_associated_with::c-Fos transcription, a mediator of light sensitivity, significantly drops. In recent years, data have supported is_associated_with::melanopsin as the main circadian photoreceptor, in particular melanopsin cells that mediate entrainment and communication between the is_associated_with::eye and the suprachiasmatic nucleus (SCN). One of the main difficulties in confirming or denying CRY as a mammalian photoreceptor is that when the gene is knocked out the animal goes arrhythmic, so it is hard to measure its capacity as purely a photoreceptor. However, some recent studies indicate that human CRY may mediate light response in peripheral tissues.

Normal mammalian circadian rhythm relies critically on delayed expression of Cry1 following activation of the Cry1 promoter. Whereas rhythms in Per2 promoter activation and Per2 mRNA levels have almost the same phase, Cry1 mRNA production is delayed by approximately four hours relative to Cry1 promoter activation. This delay is independent of CRY1 or CRY2 levels and is mediated by a combination of E/E’-box and D-box elements in the promoter and RevErbA/ROR binding elements (RREs) in the gene’s first intron. is_associated_with::Transfection of arrhythmic Cry1−/− Cry2−/− double-knockout cells with only the Cry1 promoter (causing constitutive Cry1 expression) is not sufficient to rescue rhythmicity. Transfection of these cells with both the promoter and the first intron is required for restoration of circadian rhythms in these cells.

Magnetoception
Cryptochromes in the photoreceptor neurons of birds' eyes are involved in magnetic orientation during migration. Cryptochromes are also essential for the light-dependent ability of is_associated_with::Drosophila to sense is_associated_with::magnetic fields. Magnetic fields were once reported to affect cryptochromes also in Arabidopsis thaliana plants: growth behavior seemed to be affected by magnetic fields in the presence of blue (but not red) light. Nevertheless, these results have later turned out to be irreproducible under strictly controlled conditions in another laboratory, showing that plant cryptochromes do not respond to magnetic fields.

According to one model, cryptochrome forms a pair of radicals with correlated spins when exposed to blue light. The occurrence of such light-generated radical pairs and the correlation of the radical pair state have been confirmed recently in a cryptochrome of is_associated_with::Xenopus laevis. However, recent evidence also suggests that radical pairs can be generated by the light-independent dark reoxidation of Flavin protein by molecular oxygen through the formation of a spin-correlated FADH-superoxide radical pairs. Magnetoception is hypothesized to function through the surrounding magnetic field's effect on the correlation (parallel or anti-parallel) of these radicals, which affects the duration that cryptochrome remains activated. Activation of cryptochrome may affect the light-sensitivity of is_associated_with::retinal neurons, with the overall result that the animal can "see" the magnetic field.