PIAS3

E3 SUMO-protein ligase PIAS3 is an is_associated_with::enzyme that in humans is encoded by the PIAS3 is_associated_with::gene. PIAS proteins were originally identified in studies that were aimed to decipher the Janus Kinase (JAK)/STAT signaling pathway. The mammalian PIAS family consists of four members: PIAS1, PIAS2, PIAS3 and PIAS4. In Drosophila, a single PIAS homologue named dPIAS/Zimp has been identified. In yeast, two PIAS-related proteins were identified namely SIZ1 and SIZ2. Studies by different research groups have now identified a rapidly growing list of more than 60 proteins, most of them transcription factors that can be either positively or negatively regulated by members of the PIAS family through multiple mechanisms. Originally, PIAS3 was found to interact specifically with phosphorylated STAT3 in Interleukin -6 (IL-6) activated murine myeloblast M1 cells. This interaction is mediated via PIAS3 binding to the STAT3 DNA binding domain. Hence, STAT3 transcriptional activity is inhibited by the physical prevention of its binding to target genes. Subsequently, PIAS3 was also found to be a regulator protein of other key transcription factors, including is_associated_with::MITF, is_associated_with::NFκB, is_associated_with::SMAD and estrogen receptor.

Domains
The SAF-A/B, Acinus and PIAS (SAP) domain is located at the N-terminal of PIAS proteins. This evolutionarily conserved domain is found in proteins ranging from yeast to human and is shared by other chromatin-binding proteins, such as scaffold attachment factor A and B. The SAP domain can recognize and bind to AT-rich DNA sequences present in scaffold-attachment regions/matrix-attachment regions. These elements are frequently found near gene enhancers and interact with nuclear matrix proteins to provide a unique nuclear microenvironment for transcriptional regulation. An LXXLL signature motif is present within the SAP domain of all PIAS proteins. This signature motif has been shown to mediate interactions between nuclear receptors and their co-regulators. It is also essential for the binding of PIAS3 to androgen receptor. The LXXLL motif represents the minimal requirement for the interaction with the NFκB p65 subunit and for the inhibition of NFκB transcriptional activity. It was previously described that the LXXLL motif is also responsible for the retention of PIAS3 in the nucleus. The Pro-Ile-Asn-Ile-Thr (PINIT) motif represents a highly conserved region of PIAS proteins, which was shown to be involved in the nuclear retention of PIAS3. Within the PINIT domain, the PIAS382-132 region was isolated and characterized as an inhibitory domain that binds and inhibits both the MITF and STAT3 transcription factors. The RING-finger-like zinc-binding domain (RLD) is one of the most conserved domains of the PIAS family and has been shown to be important for PIAS3 activity as a SUMO-E3 ligase. The RLD domain is also involved in the positive regulation of SMAD3 by PIAS3.

Interactions
PIAS3 has been shown to interact with is_associated_with::GFI1, is_associated_with::RELA, is_associated_with::Mothers against decapentaplegic homolog 3, is_associated_with::Microphthalmia-associated transcription factor, is_associated_with::HMGA2 and is_associated_with::Mothers against decapentaplegic homolog 2.

Related gene problems

 * is_associated_with::TAR syndrome
 * is_associated_with::1q21.1 deletion syndrome
 * is_associated_with::1q21.1 duplication syndrome