FADD


 * This article is about molecular biology. For other uses, see is_associated_with::Fadd (disambiguation).

Fas-Associated protein with Death Domain (FADD), also called MORT1, is encoded by the FADD gene on the 11q13.3 region of chromosome 11 in humans.

FADD is an adaptor protein that bridges members of the tumor necrosis factor receptor superfamily, such as the Fas-receptor, to procaspases 8 and 10 to form the is_associated_with::death-inducing signaling complex (DISC) during is_associated_with::apoptosis. As well as its most well known role in apoptosis, FADD has also been seen to play a role in other processes including proliferation, cell cycle regulation and development.

Structure
FADD is a 23 kDa protein, made up of 280 amino acids. It contains two main domains: a C terminal is_associated_with::death domain (DD) and an N terminal is_associated_with::death effector domain (DED). Each domain, although sharing very little sequence similarity, are structurally similar to one another, with each consisting of 6 α helices. The DD of FADD binds to receptors such as the Fas receptor at the plasma membrane via their DD. The interaction between the death domains are is_associated_with::electrostatic interactions involving α helices 2 and 3 of the 6 helix domain. The DED binds to the DED of intracellular molecules such as procaspase 8. It is thought that this interaction occurs through hydrophobic interactions.

Extrinsic apoptosis
Upon stimulation by the is_associated_with::Fas ligand, the Fas receptor trimerises. Many receptors, including Fas, contain a cytoplasmic DD and are therefore named death receptors. FADD binds to the DD of this trimeric structure via its death domain resulting in unmasking of FADD's DED and subsequent recruitment of procaspase 8 and 10 via an interaction between the DEDs of both FADD and the procaspases. This generates a complex known as the death inducing signalling complex (DISC). Procaspase 8 and 10 are known as initiator is_associated_with::caspases. These are inactive molecules, but when bought into close proximity with other procaspases of the same type, autocatalytic cleavage occurs at an aspartate residue within their own structures, resulting in an activated protein. This activated protein can then go on to cleave and activate further caspases, initiating the caspase cascade. The activated caspases can go on to cleave intracellular proteins such as inhibitor of caspase-activated DNase (ICAD), which ultimately leads to apoptosis of the cell.

Binding of is_associated_with::TRAIL to death receptors four and five (DR4 and DR5) can lead to apoptosis by the same mechanism.

Apoptosis can also be triggered by binding of a ligand to tumor necrosis factor receptor 1 (TNFR1); however, the mechanism by which this occurs is slightly more complex. Another DD-containing adaptor protein named is_associated_with::TRADD, along with other proteins, binds to activated TNF1R, forming what is known as complex I. This results in activation of the is_associated_with::NFκB pathway, which promotes cell survival. This complex is then internalised, and FADD binds to TRADD via an interaction of the DD’s of the two adapter proteins, forming what is known as complex II. FADD again recruits procaspase 8, which initiates the caspase cascade leading to apoptosis.



Necroptosis
FADD also plays a role in regulating is_associated_with::necroptosis, a process requiring the serine/threonine kinases, is_associated_with::RIPK1 and is_associated_with::RIPK3. Activated caspase 8 cleaves these is_associated_with::kinases, inhibiting necroptosis. Since activation of caspase 8 requires FADD in order to bring the procaspase 8 molecules into close proximity to one another to facilitate their activation, FADD is required for negatively regulating necroptosis. In accordance, cells deficient in FADD induce necroptosis as they are unable to recruit and activate procaspase 8. FADD can also bind to RIPK1 and RIPK3 directly, however the significance of this interaction is currently unclear.

Autophagic cell death
is_associated_with::Autophagy is a process which allows cell survival under stressed conditions but can also lead to cell death.

Using its DD, FADD interacts with is_associated_with::ATG5, a protein involved in autophagy. This interaction has been shown to be essential for autophagic cell death, which is induced by is_associated_with::IFN-γ. In contrast, it has also been found to inhibit autophagic cell death and therefore promote cell survival. FADD binds to ATG5 in a complex which also contains is_associated_with::ATG12, Caspase 8 and RIPK1. The formation of this complex is stimulated by autophagic signalling. Caspase 8 then cleaves RIPK1, leading to inhibition of this signalling, inhibiting cell death.

Development
FADD knockout in mouse embryos is lethal, showing a role for FADD in embryonic development. This is thought to be due to abnormal development of the is_associated_with::heart. This abnormal heart development may be due to FADD dependent regulation of the NFκB pathway.

FADD also plays a role in the development of the is_associated_with::eyes of is_associated_with::zebrafish.

Cell cycle regulation
FADD is thought to have a role in regulating the cell cycle of T lymphocytes. This regulation is dependent on phosphorylation of FADD on Serine 194, which is carried out by Casein Kinase 1a (CKIα). This phosphorylated form of FADD is found mainly in the nucleus and the abundance of phosphorylated FADD increases significantly in the is_associated_with::G2 phase of the is_associated_with::cell cycle compared to the is_associated_with::G1 phase where only very little can be detected. As it is found at the mitotic spindle during G2, it has been proposed to mediate the G2/M transition, however, the mechanism by which it does this it not yet known.

Lymphocyte proliferation
FADD is essential for is_associated_with::T cell proliferation when the is_associated_with::T cell receptor is stimulated by is_associated_with::antigen. In contrast, FADD has no effect on the proliferation of is_associated_with::B cells induced by stimulation of the is_associated_with::B cell receptor. However, it is required for B cell proliferation induced by stimulation of is_associated_with::TLR3 and is_associated_with::TLR4.

Inflammation
Activation of nuclear factor kappa B (NFκB) signalling leads to transcription of various is_associated_with::proinflammatory cytokines as well as anti-apoptotic genes. It was found that NFκB signalling was inhibited in FADD-deficient cells after stimulation of the TNF-R1 or Fas receptors. This suggests a role of FADD in activation of the NFκB pathway. Conversely, FADD has also has a role in inhibition of this pathway. Normally, upon stimulation of the receptors TL4 or IL-1R1, the adaptor protein, is_associated_with::MyD88, is recruited to the plasma membrane where is binds to IL-1 receptor associated Kinase (IRAK) via a DD-DD interaction. This activates a signalling pathway which results in translocation of NFκB to the nucleus, where it induces the transcription of the inflammatory cytokines. FADD can interfere with the interaction between MyD88 and IRAK, by binding to MyD88 via its DD and therefore this disrupts the cascade which would lead to NFκB translocation and inflammation.

Other
FADD is required for an efficient antiviral response. Upon viral infection, FADD is needed to increase the levels of Irf7 a molecule which is needed for the production of IFN-α. IFN-α is a key molecule involved in the response against is_associated_with::viruses.

FADD is involved in the activation of the is_associated_with::phosphatases which dephosphorylate and deactivate is_associated_with::Protein Kinase C (PKC). Without FADD, PKC remains active and is able to continue signalling cascades leading to processes including cytoskeletal rearrangements and cell motility.

Recent research has also shown that it may have a role in regulating is_associated_with::glucose levels and the phosphorylated form of FADD is important for this function.

Subcellular localisation
FADD can be found in both the nucleus and is_associated_with::cytoplasm of cells. Phosphorylation of Ser194 of FADD in humans (or Ser191 in mice) is thought to regulate its subcellular localisation. A is_associated_with::nuclear localization sequence and is_associated_with::nuclear export signal, both located in the DED of FADD, are also required for it to enter and exit the nucleus. Depending on its is_associated_with::subcellular localisation, FADD can have different roles. In the cytoplasm, its main function is to induce apoptosis. However, in the nucleus, it can have the opposite effect and instead promote survival.

c-FLIP
Cellular FLICE inhibitory protein (c-FLIP) is a regulatory protein which contains two DEDs. There are two isoforms of C-FLIP: C-FLIPS and FLIPL. It was originally thought to act as a negative regulator of apoptosis by binding to the DED of FADD and therefore preventing procaspase 8 from binding and inhibiting formation of the DISC. However, it has been seen that both c-FLIP and procaspase 8 can be found at the same DISC. Therefore it has been proposed that the presence of c-FLIP inhibits the close interaction of the procaspases to one another. Without this close proximity, the procaspases cannot be completely cleaved and remain in an inactive state.

PKC
The activity of protein kinase C has a negative effect on Fas receptor mediated apoptosis. This is because it inhibits the recruitment of FADD to the receptor and so a DISC is not formed. It has been shown that by either increasing or decreasing the amount of PKC in T cells, more or less FADD is recruited to FasR respectively, when the FasR is stimulated.

MKRN1
MKRN1 is an E3 ubiquitin ligase which negatively regulates FADD by targeting it for ubiquitin mediated degradation. In doing so, MKRN1 is able to control the level of apoptosis.

Roles in inflammatory diseases
Increased levels of FADD were found in the leukocytes of patients with relapsing remitting is_associated_with::multiple sclerosis, contributing to is_associated_with::inflammation. In is_associated_with::rheumatoid arthritis, it is thought that stimulation of Fas receptors on is_associated_with::macrophages, leads to formation of the FADD containing DISCs. Formation of these sequesters FADD away from MyD88 allowing MyD88 to interact with IRAK and induce the enhanced inflammation associated with this disease.

Roles in Cancer
As FADD has such an important role in apoptosis, loss of FADD can give cancer cells a proliferative advantage as apoptosis would no longer be induced when the Fas receptors are stimulated.

However, there is significant upregulation of FADD in head and neck is_associated_with::squamous cell carcinoma. It is not yet clear what advantage this has on the cancer cells, but given FADDs roles in cell cycle regulation and cell survival, it likely that it may be related to this. There are also elevated levels of FADD in non small cell lung cancer. FADD can be used as a is_associated_with::prognosis marker for both of these diseases, with high levels of FADD being correlated with poor outcome.

Therapeutic Target
Taxol is a drug used in anticancer therapies due to its ability to interfere with is_associated_with::microtubule assembly, which leads to cell cycle arrest. FADD phosphorylated at Ser194 makes cells more sensitive to cell cycle arrest induced by taxol. Taxol can also cause apoptosis of cells and this requires procaspase 10, which is activated by recruitment to FADD.

It has been shown that the activation of JNK leads to the phosphorylation of FADD. Phosphorylated FADD can induce G2/M cell cycle arrest, potentially by increasing the stability of p53. Therefore drugs which can activate this pathway may have a therapeutic potential. However, high levels of phosphorylated FADD have been correlated with a poor prognosis in many cancers such as that of the head and neck. This is likely to be due to its activation of the NF-κB pathway, which is antiapoptotic. Therefore, inhibition of FADD phosphorylation may be developed as a potential anti cancer strategy.

Interactions
FADD has been seen to interact with is_associated_with::Fas receptor,:


 * is_associated_with::ABCA1,
 * is_associated_with::ATG5,
 * C-FLIP, MKRN1,
 * Casein Kinase 1a,
 * is_associated_with::DEDD,
 * is_associated_with::MBD4
 * MyD88,
 * NACA,
 * is_associated_with::PEA15,
 * is_associated_with::RIPK1,
 * is_associated_with::RIPK3,
 * is_associated_with::TRADD,
 * is_associated_with::TRAIL,
 * procaspase 10, and
 * Procaspase 8.