Glycogen debranching enzyme



A debranching enzyme is a molecule that helps facilitate the breakdown of is_associated_with::glycogen, which serves as a store of glucose in the body, through glucosyltransferase and glucosidase activity. Together with is_associated_with::phosphorylases, debranching enzymes mobilize is_associated_with::glucose reserves from glycogen deposits in the muscles and liver. This constitutes a major source of energy reserves in most organisms. Glycogen breakdown is highly regulated in the body, especially in the is_associated_with::liver, by various hormones including is_associated_with::insulin and is_associated_with::glucagon, to maintain a homeostatic balance of blood-glucose levels. When glycogen breakdown is compromised by mutations in the glycogen debranching enzyme, metabolic diseases such as is_associated_with::Glycogen storage disease type III can result.

Glucosyltransferase and glucosidase are performed by a single is_associated_with::enzyme in mammals, yeast, and some bacteria, but by two distinct enzymes in is_associated_with::E. coli and other bacteria, complicating nomenclature. Proteins that catalyze both functions are referred to as glycogen debranching enzymes (GDEs). When glucosyltransferase and glucosidase are catalyzed by distinct enzymes, "glycogen debranching enzyme" usually refers to the glucosidase is_associated_with::enzyme. In some literature, an enzyme capable only of glucosidase is referred to as a "debranching enzyme".

Function
Together with is_associated_with::phosphorylase, glycogen debranching enzymes function in glycogen breakdown and glucose mobilization. When phosphorylase has digested a glycogen branch down to four glucose residues, it will not remove further residues. Glycogen debranching enzymes assist is_associated_with::phosphorylase, the primary enzyme involved in is_associated_with::glycogen breakdown, mobilize glycogen stores. Phosphorylase can only cleave α-1,4- glycosidic bond between adjacent glucose molecules in glycogen but branches exist as α-1,6 linkages. When phosphorylase reaches four residues from a branching point it stops cleaving; because 1 in 10 residues is branched, cleavage by phosphorylase alone would not be sufficient in mobilizing glycogen stores. Before phosphorylase can resume catabolism, debranching enzymes perform two functions:


 * 4-α-D-glucanotransferase, or is_associated_with::glucosyltransferase, transfers three glucose residues from the four-residue glycogen branch to a nearby branch. This exposes a single glucose residue joined to the glucose chain through an α -1,6 glycosidic linkage


 * Amylo-α-1,6-glucosidase, or is_associated_with::glucosidase, cleaves the remaining alpha-1,6 linkage, producing glucose and a linear chain of glycogen. The mechanism by which the glucosidase cleaves the α -1,6-linkage is not fully known because the is_associated_with::amino acids in the is_associated_with::active site have not yet been identified. It is thought to proceed through a two step acid base assistance type mechanism, with an is_associated_with::oxocarbenium ion intermediate, and retention of configuration in glucose. This is a common method through which to cleave bonds, with an acid below the site of is_associated_with::hydrolysis to lend a proton and a base above to deprotinate a water which can then act as a is_associated_with::nucleophile. These acids and bases are amino acid side chains in the active site of the enzyme. A scheme for the mechanism is shown in the figure below.



Thus the debranching enzymes, transferase and α-1,6- glucosidase converts the branched glycogen structure into a linear one, paving the way for further cleavage by phosphorylase.

Two enzymes
In E. coli and other bacteria, glucosyltransferase and glucosidase functions are performed by two distinct enzymes. In E. coli, Glucose transfer is performed by 4-alpha-glucanotransferase, a 78.5 kDa protein coded for by the gene malQ. A second protein, referred to as debranching enzyme, performs α-1,6-glucose cleavage. This enzyme has a molecular mass of 73.6 kDa, and is coded for by the gene glgX. Activity of the two enzymes is not always necessarily coupled. In E. coli glgX selectively catalyzes the cleavage of 4-subunit branches, without the action of glucanotransferase. The product of this cleavage, is_associated_with::maltotetraose, is further degraded by maltodextrin phosphorylase.

E. coli GlgX is structurally similar to the protein is_associated_with::isoamylase. The monomeric protein contains a central domain in which eight parallel beta-strands are surrounded by eight parallel alpha strands. Notable within this structure is a groove 26 angstroms long and 9 angstroms wide, containing aromatic residues that are thought to stabilize a four-glucose branch before cleavage.

One enzyme with two catalytic sites
In mammals and is_associated_with::yeast, a single enzyme performs both debranching functions. The human glycogen debranching enzyme (gene: AGL) is a monomer with a molecular weight of 175 kDa. It has been shown that the two catalytic actions of AGL can function independently of each other, demonstrating that multiple active sites are present. This idea has been reinforced with inhibitors of the active site, such as polyhydroxyamine, which were found to inhibit glucosidase activity while transferase activity was not measurably changed. Glycogen debranching enzyme is the only known eukaryotic enzyme that contains multiple catalytic sites and is active as a monomer.

Some studies have shown that the C-terminal half of yeast GDE is associated with glucosidase activity, while the N-terminal half is associated with glucosyltransferase activity. In addition to these two is_associated_with::active sites, AGL appears to contain a third active site that allows it to bind to a glycogen polymer. Though the complete structure of the GDE in Eukaryotes is yet to be determined it is thought to bind to six glucose molecules of the chain as well as the branched glucose, thus corresponding to 7 subunits within the active site, as shown in the figure below.



It was seen that when glucose ‘a’, ‘b’, ‘c’ and ‘0’ in the is_associated_with::active site was hydrolyzed the most rapidly. This indicated that this region of the glycogen chain bond strongest to the active site because a stronger interaction between enzyme and substrate leads to a more rapid hydrolysis.

Despite these advances, the complete structure of GDE in eukaryotes has yet to be determined. The glycogen-degrading enzyme of the is_associated_with::archaea is_associated_with::Sulfolobus solfataricus is better characterized than those of is_associated_with::eukaryotes. The GDE of S. solfataricus is known as treX. Although, like mammalian GDE, treX has both amylosidase and glucanotransferase functions, TreX is structurally similar to glgX, and hass a mass of 80kD and one active site. Unlike either glgX or AGL, however, treX exists as a dimer and tetramer in solution. TreX's oligomeric form seems to play a significant role in altering both enzyme shape and function. Dimerization is thought to stabilize a "flexible loop" located close to the active site. This may be key to explaining why treX (and not glgX) shows glucosyltransferase activity. As a tetramer, the catalytic efficiency of treX is increased fourfold over its dimeric form.

Genetic Location
The official name for the gene is “amylo- α- 1,6- glucosidase, 4- α- glucanotransferase,” with the official symbol AGL. AGL is an autosomal gene found on chromosome lp21. The AGL gene provides instructions for making several different versions, known as isoforms, of the glycogen debranching enzyme. These isoforms vary by size and are expressed in different tissues, such as liver and muscle. This gene has been studied in great detail, because mutation at this gene is the cause of Glycogen Storage Disease Type III. The gene is 85 kb long, has 35 is_associated_with::exons and encodes for a 7.0 kb- mRNA. Translation of the gene begins at exon 3,which encodes for the first 27 amino acids of the AGL gene, because the first two exons (68kb) contain the 5’ untranslated region. Exons 4-35 encode the remaining 1505 amino acids of the AGL gene. Studies produced by the department of pediatrics at Duke University suggest that the human AGL gene contains at minimum 2 promotor regions, sites where the transcription of the gene begins, that result in differential expression of isoform, different forms of the same protein, mRNAs in a manner that is specific for different tissues.

Pathology
When GDE activity is compromised, the body cannot effectively release stored glycogen, type III Glycogen Storage Disease (debrancher deficiency), an autosomal recessive disorder, can result. In GSD III glycogen breakdown is incomplete and there is accumulation of abnormal glycogen with short outer branches.

Most patients exhibit GDE defiency in both liver and muscle (TypeIIIa), although 15% of patients have retained GDE in muscle while having it absent from the liver (Type IIIb). Depending on is_associated_with::mutation location, different mutations in the AGL gene can affect different isoforms of the is_associated_with::gene expression. For example, mutations that occur on exon 3, affect the form which affect the is_associated_with::isoform that is primarily expressed in the liver; this would lead to GSD type III.

These different manifestation produce varied symptoms, which can be nearly indistinguishable from Type I GSD, including is_associated_with::hepatomegaly, is_associated_with::hypoglycemia in children, short stature, is_associated_with::myopathy, and is_associated_with::cardiomyopathy. Type IIIa patients often exhibit symptoms related to liver disease and progressive muscle involvement, with variations caused by age of onset, rate of disease progression and severity. Patients with Type IIIb generally symptoms related to liver disease. Type III patients be distinguished by elevated liver enzymes, with normal is_associated_with::uric acid and blood lactate levels, differing from other forms of GSD. In patients with muscle involvement, Type IIIa, the muscle weakness becomes predominant into adulthood and can lead to ventricular is_associated_with::hypertrophy and distal muscle wasting.