PGRMC1

Progesterone receptor membrane component 1 (abbreviated PGRMC1) is a is_associated_with::protein which co-purifies with progesterone binding proteins in the liver and ovary. In humans, the PGRMC1 protein is encoded by the PGRMC1 is_associated_with::gene. The is_associated_with::Sigma-2 receptor was recently identified as potentially being the same as PGRMC1.

The sole biochemical function of PGRMC1 is is_associated_with::heme-binding. PGRMC1 shares key structural motifs with cytochrome b5. PGRMC1 binds and activates P450 proteins,  which are important in drug, hormone and lipid metabolism. PGRMC1 also binds to PAIR-BP1 (plasminogen activator inhibitor RNA-binding protein-1). However, its expression outside of the reproductive tract and in males suggests multiple functions for the protein. These may include binding to Insig (insulin-induced gene), which regulates cholesterol synthesis.

Expression
PGRMC1 is highly expressed in the liver and kidney in humans with lower expression in the brain, lung, heart, skeletal muscle and pancreas. In rodents, PGRMC1 is found in the liver, lung, kidney and brain. PGRMC1 is over-expressed in breast tumors and in cancer cell lines from the colon, thyroid, ovary, lung, and cervix. is_associated_with::Microarray analyses have detected PGRMC1 expression in colon, lung and breast tumors.

PGRMC1 expression is induced by the non-genotoxic is_associated_with::carcinogen 2,3,7,8-tetrachlorodibenzo-p-dioxin in the rat liver, but this induction is specific to males. PGRMC1 is expressed in the ovary and is_associated_with::corpus luteum, where its expression is induced by progesterone and during pregnancy, respectively. PGRMC1/25-Dx is expressed in various regions of the brain [hypothalamic area, circumventricular organs, ependymal cells of the lateral ventricles, meninges, including regions known to facilitate is_associated_with::lordosis.

PGRMC1 binding to heme and P450s is conserved from yeast to humans
The PGRMC1 yeast homologue, Dap1 (damage associated protein 1), binds heme through a penta-coordinate mechanism. Yeast cells lacking the DAP1 gene are sensitive to DNA damage, and heme-binding is essential for damage resistance. Dap1 is also required for a critical step in cholesterol synthesis in which the P450 protein Erg11/Cyp51 removes a methyl group from lanosterol. Erg11/Cyp51 is the target of the azole antifungal drugs. As a result, yeast cells lacking the DAP1 gene are highly sensitive to antifungal drugs  This function is conserved between the unrelated fungi S. cerevisiae and S. pombe. Dap1 also regulates the metabolism of iron in yeast.

In yeast and humans, PGRMC1 binds directly to P450 proteins, including is_associated_with::CYP51A1, is_associated_with::CYP3A4, is_associated_with::CYP7A1 and is_associated_with::CYP21A2. PGRMC1 also activates Cyp21 when the two proteins are co-expressed, indicating that PGRMC1 promotes progesterone turnover. Just as Dap1 is required for the action of Erg11 in the synthesis of ergosterol in yeast, PGRMC1 regulates the Cyp51-catalyzed demethylation step in human cholesterol synthesis. Thus, PGRMC1 and its homologues bind and regulate P450 proteins, and it has been likened to “a helping hand for P450 proteins”.

Roles in signaling and apoptosis
The yeast PGRMC1 homologue is required for resistance to damage. PGRMC1 also promotes survival in human cancer cells after treatment with chemotherapy. In contrast, PGRMC1 promotes cell death in cancer cells after oxidative damage. PGRMC1 alters several known survival signaling proteins, including the is_associated_with::Akt is_associated_with::protein kinase and the cell death-associated protein is_associated_with::IκB. Progesterone inhibits apoptosis in immortalized is_associated_with::granulosa cells, and this activity requires PGRMC1 and its binding partner, PAIR-BP1 (plasminogen activator inhibitor RNA-binding protein-1). However, PAIR-BP1 is not a progesterone binding protein, and the component of the PGRMC1 complex that binds to progesterone is unknown.