C7orf30

C7ORF30 (chromosome 7 is_associated_with::open reading frame 30) is a is_associated_with::gene on is_associated_with::chromosome 7 in humans that encodes the is_associated_with::protein LOC_115416. This protein localizes to mitochondria and is probably involved in mitochondrial translation or the is_associated_with::biogenesis of the large subunit of the is_associated_with::mitochondrial ribosome.

Protein
LOC_115416 is a member of the DUF143 family (= is_associated_with::domain of unknown function 143, ) which is highly conserved in both is_associated_with::prokaryotes and is_associated_with::eukaryotes but not is_associated_with::archaea. Examples of mammalian conservation are given below using the ALIGN tool from the is_associated_with::San Diego Supercomputer Center Biology Workbench. Percentages indicate the identity shared by the human protein and the respective mammalian protein. Accession numbers are from the NCBI database.

There are no known or predicted paralogs in is_associated_with::Homo sapiens. That is, C7Orf30 is a single-copy gene.

The domain is from position 93 to 194 on the human protein and comprises 43.2% of the sequence. This conserved domain is also present in the plant gene is_associated_with::iojap, a pattern-striping gene in maize. However, since its function has been solved at least in bacteria, it is no longer a "domain of unknown function".

Protein function
While the function of the protein in mitochondria is not conclusive its bacterial homolog has been shown to silence is_associated_with::prokaryotic translation by blocking the two is_associated_with::ribosomal is_associated_with::subunits from joining, hence it was called RsfS (= ribosomal silencing factor in starvation or stationary phase, a synonym of RsfA ).

Protein-protein interactions. RsfS has been shown via a yeast two-hybrid screen to interact with ribosomal protein L14 in four bacterial species as well as in mitochondria. C7Orf30 was shown to interact with CHMP protein which is part of the is_associated_with::ESCRT-III complex (Endosomal Sorting Complex Required for Transport). DUF143 has also been shown to interact with UFD1, tRNA synthetases class II, and Cytidylyltransferase in various architectures.

Properties
is_associated_with::Bioinformatics predicted the following properties for LOC_115416:
 * Molecular Weight: 26.2 KDal
 * Isoelectric Point: 5.155

Gene
C7ORF30 is located on chromosome 7 in humans and runs from 23,305,465 to 23,315,705. There are four predicted exons in the human gene with conservation occurring across most mammalian species. There is no conclusive data regarding whether the gene is ubiquitously expressed in human tissues, but is_associated_with::expressed sequence tag databases show that it is expressed in many tissues.

Neighboring Genes
C7ORF30 is neighbored by is_associated_with::GPNMB upstream and is_associated_with::IGF2BP3 downstream, however the latter gene is transcribed on the opposite strand running from the 3' to the 5' end. There is some slight overlap of the untranslated regions of C7ORF30 and IGF2BP3 whereas the distance between C7ORF30 and GPNMB is 24,211 base pairs.