Transducin



Transducin (also called Gt) is a heterotrimeric G protein that is naturally expressed in vertebrate retina rods and cones (a different Transducin gene is expressed in each cell type).

Mechanism of action
Heterotrimeric Transducin (alpha-beta-gamma subunits) is activated by a conformational change in rhodopsin due to the absorption of a photon by rhodopsin's active group retinal. Activation causes the GDP bound to the alpha subunit to be exchanged with GTP from solution and results in activated alpha dissociating from beta-gamma. Active Transducin-alpha then causes cyclic GMP Phosphodiesterase to increase its activity, thereby lowering the concentration of cGMP, an intracellular second-messenger molecule. Decrease in cGMP concentration leads to the closure of cGMP-regulated Na+ and Ca2+ ion channels and a hyperpolarized membrane potential. This chain of signaling events is also called "the vertebrate phototransduction cascade".

The termination of Transducin activity occurs when (active) GTP bound Transducin is hydrolyzed to Transducin-GDP, a process which is accelerated by a complex containing an RGS (Regulator of G-protein signaling)-protein and the gamma-subunit of the effector, cyclic GMP Phosphodiesterase.